Avaliação de diferentes métodos de preservação de culturas fúngicas

Evaluation of different methods for preservation of fungal cultures

Ceci Sales-Campos, Kelly Magalhães Gonçalves, Maria Aparecida de Jesus, Meire Cristina Nogueira de Andrade

Resumo


O contínuo isolamento de culturas e a necessidade de sua manutenção para estudos acerca do cultivo, produtividade, caracterização nutricional, biotecnológica, tanto para objetivos acadêmicos como para fins comerciais e industriais são de fundamental importância. Assim, o objetivo deste trabalho foi avaliar diferentes métodos de preservação de culturas fúngicas, com o propósito de pesquisas acerca do cultivo de cogumelos e estudos afins. Para verificar a eficiência dos métodos, foi feita uma avaliação após seis meses de preservação. Para isso, foram utilizados dois tubos das culturas mantidas em diferentes métodos (água destilada, em óleo mineral, repicagens periódicas e sílica gel). Fragmentos dessas culturas foram novamente inoculadas em placas de Petri contendo meio malte agar (3%). Foram feitas quatro repetições (placas) por linhagem, das quais duas foram adicionadas de serragem de marupá, com o fim de testar a eficácia dos referidos métodos. Lentinus strigosus foi o fungo que apresentou crescimento mais rápido e, nos demais métodos, cresceu em cinco dias. O período de colonização das linhagens de Pleurotus ostreatus, provenientes dos métodos de preservação em água destilada, óleo mineral e repicagem periódica foi de seis dias, sem diferença do tempo de colonização entre os métodos. A linhagem de Pleurotus sajor-caju e as linhagens de P. ostreatus colonizaram o meio em sete dias de incubação em todos os métodos, exceto em sílica gel. O tempo de colonização das linhagens de Lentinula edodes LED 25, LED 27, CHICO II, Flamulina sp. e de Pleurotus ostreatus POS 09/99 e POS 09/100 foi de nove dias de incubação. As linhagens de L. edodes LED 12, LED 20, LED 33, LED 35, LED 51, LED 55, LED 58, LED 75, LED 96/13, LED SALTO, LED JABL, LED SIII e a linhagem de Coprinus comatus CCO 01/01 obtiveram maior delonga no tempo de colonização (10 dias).

Abstract

The continual isolation of cultures and the need for their maintenance for studies on the cultivation, yield, nutritional and biotechnological characterization, both for academic and for commercial and industrial purposes are of fundamental importance. Thus, the objective of this study was to evaluate different methods of preserving fungal cultures for the purpose of research on the cultivation of mushrooms and correlated studies. To verify the efficiency of the methods, an evaluation was carried out after six months of preservation. Two test tubes were used from cultures maintained at different methods (distilled water, mineral oil and periodical transplanting and silica gel). Fragments of these cultures were once again inoculated in Petri plates containing malt agar (3%). Four replications were performed (plaques) per strain, two of which were added marupá sawdust in order to test the effectiveness of those methods. Lentinus strigosus was the fungus that experienced the fastest growth, and in other methods, it has grown in five days. The colonization period of Pleurotus ostreatus strains, using the methods of preservation in distilled water, mineral oil and periodical transference was six days, without difference in the colonization time between the methods. The P. sajor-caju strain and the P. ostreatus strains colonized the medium in seven days of incubation in all the methods, except on silica gel. The colonization time of Lentinula edodes LED 25, LED 27, CHICO II, Flamulina sp. and Pleurotus ostreatus POS 09/99 e POS 09/100 strains was nine days of incubation. However, the L. edodes LED 12, LED 20, LED 33, LED 35, LED 51, LED 55, LED 58, LED 75, LED 96/13, LED SALTO, LED JABL, LED SIII strains and Coprinus comatus CCO 01/01 strain had greater delay in the olonization of time (10 days).


Palavras-chave


sílica gel; óleo mineral; baixa temperatura; viabilidade.

Texto completo:

PDF


AMBIÊNCIA

Alameda Élio Antonio Dalla Vechia, n. 838,
Bairro Vila Carli – CEDETEG
85040-167  Guarapuava-Paraná-Brasil
ISSN 1808-0251 (Print) - Ambiência ISSN 2175-9405 (Online)

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