Germinação de esporos e desenvolvimento gametofítico de Cyathea atrovirens (Langsd. & Fisch.) Domin (Cyatheaceae) em diferentes temperaturas e fotoperíodos

Spore germination and gametophytic development of Cyathea atrovirens (Langsd...

Catiuscia Marcon, Tatieli Silveira, Débora Bender, Annette Droste

Resumo


Cyathea atrovirens (Langsd. & Fisch.) Domin (Cyatheaceae) é uma samambaia arborescente de ampla distribuição geográfica que ocorre no Nordeste, Centro-Oeste, Sudeste e Sul do Brasil, no Paraguai, na Argentina e no Uruguai. A espécie habita ambientes diversos como florestas, áreas abertas e mesmo locais impactados pela ação humana. O estudo teve por objetivo avaliar a influencia da temperatura e do fotoperíodo na germinação de esporos e no desenvolvimento de gametófitos de C. atrovirens. Esporos foram semeados em frascos contendo meio de Meyer líquido e mantidos nas temperaturas de 15, 20, 23, 26, 29 e 32±1 ˚C (Experimento 1) e nos fotoperíodos de 8, 12, 16, 24 h de luz e no escuro (Experimento 2). As maiores porcentagens de germinação (84 a 99%) foram observadas nas temperaturas de 20 a 32 °C, enquanto que em 15 °C 43% dos esporos germinaram. Em 32 °C, a maior parte dos gametófitos ainda se encontrava em estádio inicial, com clorócito e rizoide (72%), enquanto que nas temperaturas de 20 a 29 °C, 61 a 73% dos gametófitos já estavam em estádio laminar aos 28 dias. Não houve germinação no escuro. As porcentagens de germinação e de gametófitos laminares foram igualmente altas em todos os fotoperíodos com presença de luz, variando de 91 a 97% e de 74 a 87%, respectivamente. Os dados obtidos podem contribuir para o entendimento da distribuição geográfica e da ocorrência de Cyathea atrovirens em diversos habitats.

Abstract

Cyathea atrovirens (Langsd. & Fisch.) Domin (Cyatheaceae) is a widely distributed arborescent fern, grown in the Northeast, Midwest, Southeast and South of Brazil, Paraguay, Argentina and Uruguay. The species is found in different environments such as forests, open areas and even in locations impacted by human action. The study aimed to evaluate the influence of temperature and the photoperiod on spore germination and on the gametophyte development of C. atrovirens. Spores were seeded in flasks containing liquid Meyer medium and maintained at temperatures of 15, 20, 23, 26, 29 and 32±1 ˚C (Experiment 1) and at photoperiod cycles of light and dark of 8, 12, 16, 24 h (Experiment 2). The highest germination percentages (84 to 99%) were observed at temperatures of 20 to 32 °C, while at 15 °C, 43% of the spores germinated. At 32 °C, most gametophytes still remained at the early stage, with chlorocyte and rhizoid (72%), whereas at temperatures from 20 to 29 °C, 61 to 73% of the gametophytes were already in laminar stage at 28 days. There was no germination in the dark. The percentages of germination and laminar gametophytes were equally high in all photoperiods with the presence of light, ranging from 91 to 97% and from 74 to 87%, respectively. The data obtained may contribute to the understanding of the geographical distribution and the occurrence of Cyathea atrovirens in diverse habitats.


Palavras-chave


desenvolvimento; fatores abióticos; samambaia arborescente; gametófito; reprodução.

Texto completo:

PDF


AMBIÊNCIA

Alameda Élio Antonio Dalla Vechia, n. 838,
Bairro Vila Carli – CEDETEG
85040-167  Guarapuava-Paraná-Brasil
ISSN 1808-0251 (Print) - Ambiência ISSN 2175-9405 (Online)

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Periódicos Qualis - Plataforma Sucupira

Qualis B2 - Ensino

Qualis B3 - Interdisciplinar

Qualis B3 - Planejamento Urbano e Regional / Demografia

Qualis B2 - GEOGRAFIA

InfoBase Index - IBI Factor 2016 = 2,5


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                        Copyright © 2005-2018 Editora Unicentro
Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 Unported License.
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